妊娠山羊子宫uNK细胞与VEGF动态分布研究

doi:10.11843/j.issn.0366-6964.2014.05.021

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (5): 821-826.doi: 10.11843/j.issn.0366-6964.2014.05.021

妊娠山羊子宫uNK细胞与VEGF动态分布研究

丁培阳，刘艳利，曹阳坡，李笑，靳亚平，王爱华，齐雪峰^*

（西北农林科技大学动物医学院，杨凌 712100）

收稿日期:2013-06-26 出版日期:2014-05-23 发布日期:2014-05-23
通讯作者: 齐雪峰，副教授，博士，E-mail：yxyan2002@126.com
作者简介:丁培阳(1990-)，男，河南舞阳人，本科生，主要从事家畜内分泌学与免疫学相关研究，E-mail：dingpeiyang@nwsuaf.edu.cn
基金资助:
中央直属高校科研基本业务费（ZD2013008）；西北农林科技大学大学生创新创业训练计划项目(1201210712033)

Study of the Dynamic Distribution of uNK Cells and VEGF in the Pregnant Goat

DING Pei-yang，LIU Yan-li，CAO Yang-po，LI Xiao，JIN Ya-ping，WANG Ai-hua，QI Xue-feng^*

（College of Veterinary Medicine，Northwest A &F University ，Yangling 712100， China）

Received:2013-06-26 Online:2014-05-23 Published:2014-05-23

摘要/Abstract

摘要：

探讨子宫内自然杀伤细胞（uNK）及血管内皮细胞生长因子（VEGF）在正常妊娠山羊子宫的动态分布规律。采用免疫组织化学技术对uNK细胞和VEGF在妊娠山羊子宫内的分布以及含量的动态变化进行研究，并通过RT-PCR技术检测VEGF mRNA的动态转录水平。结果显示：在妊娠早期，uNK细胞和VEGF大量存在于蜕膜部；妊娠中期，在子宫基质、腺体和肌层血管处检测到大量uNK细胞和VEGF表达；在妊娠后期，uNK细胞量和VEGF表达急剧减少。RT-PCR技术检测显示，妊娠早期和妊娠中期VEGF mRNA的转录量差异不显著，但均显著高于妊娠晚期VEGF mRNA的转录水平（P<0.05）。山羊子宫内uNK细胞和VEGF在妊娠不同时期的分布规律呈现一定相关性。

Abstract:

The aim of this study was to investigate the relationship between the dynamic distribution of uNK cells and VEGF in uterus of the pregnant goat.The distribution and content of uNK cells and VEGF in the pregnant uterus were detected by using immunohistochemical technology，and the transcription level of VEGF mRNA was examined by RT-PCR assay.We found that during early pregnancy，uNK cells and VEGF were concentrated in deciduas; during mid trimester of pregnancy，uNK cells and VEGF were concentrated on stroma region，gland and vessel in muscle.However，the positive staining for uNK cells and VEGF was significantly decreased in late trimester of pregnancy.The results of RT-PCR showed that in early and mid trimester of pregnancy，the transcription of VEGF mRNA had no significant deviation，but significantly higher than that of VEGF mRNA in late trimester of pregnancy (P<0.05).The results suggest that the distribution and content of uNK cells and VEGF are relevant to some extent in different pregnant period.

中图分类号:

S857.21

丁培阳，刘艳利，曹阳坡，李笑，靳亚平，王爱华，齐雪峰. 妊娠山羊子宫uNK细胞与VEGF动态分布研究[J]. 畜牧兽医学报, 2014, 45(5): 821-826.

DING Pei-yang，LIU Yan-li，CAO Yang-po，LI Xiao，JIN Ya-ping，WANG Ai-hua，QI Xue-feng. Study of the Dynamic Distribution of uNK Cells and VEGF in the Pregnant Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(5): 821-826.

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妊娠山羊子宫uNK细胞与VEGF动态分布研究

Study of the Dynamic Distribution of uNK Cells and VEGF in the Pregnant Goat

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